calcium in granule in guinea

Regulation of two insulin granule populations within the …

Insulin granule trafficking is a key step of glucose-stimulated insulin secretion from pancreatic β cells. Using quantitative live cell imaging, we examined insulin granule movements within the reserve pool upon secretory stimulation in βTC3 cells. For this study, we developed a custom image analysis program that permitted automatic tracking of the individual motions of over 20,000 granules

Calcium Stores in Hippocampal Synaptic Boutons …

Calcium-Induced Calcium Release Contributes to Bouton Calcium Transients The bouton Ca 2+ transients were blocked by addition of 10 μM cadmium to the superfusate ( in artificial cerebrospinal fluid [ACSF] versus 5.4% ± 0.9% in cadmium; p < 0.03n = 2), indiing that they result from influx of Ca 2+ from the extracellular space via voltage-gated Ca 2+ channels (VGCCs).

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L-Type Calcium Channels Mediate a Slow Excitatory …

Patch pipettes were used to record whole-cell synaptic currents under voltage-clamp in dopaminergic neurons in slices of rat substantia nigra pars compacta and ventral tegmental area. We report that dihydropyridines (DHPs), L-type Ca2+ channel antagonists, depressed a slow EPSC (EPSCslow) evoked by a train of focally delivered electrical stimuli. In fact, the amplitude of the EPSCslow was

Role of N‐type calcium channels in autonomic …

Our data demonstrates that N‐type calcium channels account for all the calcium current required for cardiac autonomic neurotransmission in the guinea‐pig isolated left atrium. British Journal of Pharmacology (1999) 127 , 927–934; doi: 10.1038/sj.bjp.0702629

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Calcium channel blockers modify electrophysiological …

Calcium channel blockers modify electrophysiological effects induced by lytic granules from cytotoxic T lymphocytes in guinea pig ventricular myocytes. O Binah , G Berke , D Rosen and B F Hoffman Journal of Pharmacology and Experimental Therapeutics March 1, 1994, 268 (3) 1581-1587;

Confocal Imaging of Neuropeptide Y-pHluorin: A …

Once the granule fuses with the plasma merane and opens, its cargo is exposed to the neutral extracellular pH of 7.4, allowing the use of the pH-sensitive proteins pHluorin as a reporter 7, 14. In view of the pH sensitive nature of pHluorin and the selective expression of NPY in insulin granules, the NPY-pHluorin fusion construct can be used to study various properties of insulin granule

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Presynaptic calcium channels mediating synaptic …

In the submucosal plexus of the guinea‐pig caecum, release of noradrenaline evoked by repetitive focal stimulation with five pulses at 40 Hz appears to be mediated solely by N‐type HVA calcium channels present on extrinsic, sympathetic nerve terminals

The merane fusion events in degranulating guinea pig …

We have investigated the granule fusion events associated with exocytosis in degranulating peritoneal guinea pig eosinophils by time-resolved patch-clamp capacitance measurements using the phase detector technique. Intracellular stimulation with micromolar

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The merane fusion events in degranulating guinea pig …

In guinea pig eosinophils capaci-tance steps of 7-20 fF were observed (Nüße et al., 1990), indiive of discrete granule fusion events during the main phase of the degranulation. We have now investigated the fine structure of the capac - itance changes during In

Frontiers | Adenosine A1 Receptor-Mediated Attenuation …

By granule cell-specific deletion of the GluA2 subunit, thereby increasing calcium influx in granule cells and, in turn, increasing dendro-dendritic inhibition of mitral cells, the discrimination between related odor mixtures was accelerated in mice (Abraham et al).

Simultaneous dendritic voltage and calcium imaging and …

2018/8/23· Dendritic calcium increased roughly incrementally with each DCS spikelet (Fig. 2a: see black arrows), in agreement with CF-evoked voltage and calcium transients being predominantly mediated by

Calcium Homeostasis in Digitonin‐Permeabilized Bovine …

Calcium Homeostasis in Digitonin‐Permeabilized Bovine Chromaffin Cells Calcium Homeostasis in Digitonin‐Permeabilized Bovine Chromaffin Cells Kao, Lung‐Sen 1988-07-01 00:00:00 Abbreviations used CCCP carbonyl cyanide m‐chlorophenyl hydrazone CG chromaffin granule ER endoplasmic reticulum IP 3 inositol trisphosphate quin2 2‐{[2‐[bis(carboxymethyl)amino]‐5‐rnethylphenoxy]methyl

The importance of being subtle: small changes in calcium …

Ashok Kumar, Calcium Signaling During Brain Aging and Its Influence on the Hippocampal Synaptic Plasticity, Calcium Signaling, 10.1007/978-3-030-12457-1_39, (985-1012), (2020). Crossref Anna Pannaccione, Ilaria Piccialli, Agnese Secondo, Roselia Ciccone, Pasquale Molinaro, Francesca Boscia, Lucio Annunziato, The Na+/Ca2+exchanger in Alzheimer’s disease, Cell Calcium, …

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Expression of tryptophan 2,3-dioxygenase in mature …

2010/9/5· New granule cells are continuously generated in the dentate gyrus of the adult hippocampus. During granule cell maturation, the mechanisms that differentiate new cells not only describe the degree of cell differentiation, but also crucially regulate the progression of cell differentiation. Here, we describe a gene, tryptophan 2,3-dioxygenase (TDO), whose expression distinguishes stem cells

Platelet-activating factor stimulates cytoplasmic …

The effects of platelet-activating factor (PAF) and IL-5 on intracellular pH were investigated in human eosinophils. Purified peripheral blood eosinophils were loaded with the ratiometric fluorescent pH indior BCECF-AM ester. Stimulation of eosinophils with PAF produced time-dependent alkalinization of the cytoplasm from an initial pH of 7.1±0.04 to 7.5±0.05. A similar alkalinization

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CiteSeerX — The role of presynaptic calcium in short …

The mossy fiber synapse between dentate granule ceils and CA3 pyramidal cells in the guinea pig hippocampus shows a robust short-term synaptic enhancement. We have simultaneously measured presynaptic residual free calcium ([Ca*+],) and postsynaptic field potentials at this synapse to examine the role of [Ca2+liin this enhancement.

Ultracytochemical Localization of Calcium in the …

Ultracytochemical Localization of Calcium in the Superficial Pineal Gland of the Mongolian Gerbil Ultracytochemical Localization of Calcium in the Superficial Pineal Gland of the Mongolian Gerbil Krstić, Radivoj 1985-01-01 00:00:00 Institute of Histology and Eryology, Lausanne, Switzerland In order t o demonstrate fine localization of (:a2+ in the superficial pineal gland o f Meriones

Astragalus Granule Prevents [Ca.sup.2+] Current …

2017/1/1· Free Online Library: Astragalus Granule Prevents [Ca.sup.2+] Current Remodeling in Heart Failure by the Downregulation of CaMKII.(Research Article, Report) by "Evidence - Based Complementary and Alternative Medicine"; Health care industry Arrhythmia Care